… human suspension packaging cell line producing ecotropic retroviral MLV (PVC-211) vectors efficiently transducing murine hematopoietic stem and progenitor cells

Y van Heuvel, K Berg, T Hirch, K Winn… - Journal of virological …, 2021 - Elsevier
… Despite their inability to transduce non-cycling cells, retroviral vectors derived from MLV …
(LTRs; dotted arrows) of murine embryonic stem cell virus (MSCV) and the packaging signal Ψ …

[HTML][HTML] Ecotropic HIV-1 vectors pseudotyped with R-peptide-deleted envelope protein variants reveal improved gene transfer efficiencies

N Tschorn, C Söhngen, K Berg, J Stitz - Virology, 2022 - Elsevier
… To elevate gene transduction, retroviral vectors are commonly … HSCs and progenitor cells
mediated by ecotropic MLV vectors … To improve gene transduction efficiencies, retroviral Env …

Development of transposon and retrovirus-derived vectors for the rapid establishment of efficient producer cell lines

N Tschorn - 2023 - repo.uni-hannover.de
… this, viral vectors were equipped with the ecotropic envelope … lines in relation to retroviral
gene transduction is provided in … optimize transposon vectors and retroviral vectors enabling …

Optimization of retroviral packaging cells for scale-up of vector production

Y van Heuvel - 2023 - repo.uni-hannover.de
… design of cell line establishment and retroviral vector- … in genomic DNA of transduced
COS7mCAT target cells. ................… ; dotted arrows) of murine embryonic stem cell virus (MSCV) and …

Construction of a replication-competent retroviral vector for expression of the VSV-G envelope glycoprotein for cancer gene therapy

SY Jin, YT Jung - Archives of Virology, 2020 - Springer
Transduction of human hematopoietic stem cells with GALV … 1, the ‘ecotropic envelope
glycoprotein’-encoding gene from … retroviral vectors in transducing human cancer cell lines. Since …

Retroviral gene therapy in Germany with a view on previous experience and future perspectives

MA Morgan, M Galla, M Grez, B Fehse, A Schambach - Gene Therapy, 2021 - nature.com
… of infectious retroviral particles (referred to as retroviral vectorstem cell virus [9], was based
on the murine embryonic stem … marrow cells transduced with alpharetroviral SIN vectors as …

Culture, differentiation, and transduction of mouse E12.5 pancreatic spheres: an in vitro model for the secondary transition of pancreas development

L Huijbregts, V Aiello, A Soggia, P Ravassard… - Islets, 2021 - Taylor & Francis
… pancreas can be specifically transduced by ALV(A)-based retroviral vectors. Using this …
transduced by ecotropic MLV-based retroviral vectors designed for either ubiquitous or beta cell

Production of retroviral vectors in continuous high cell density culture

MD Hein, D Kazenmaier, Y van Heuvel, T Dogra… - Applied Microbiology …, 2023 - Springer
… genetic modification of hematopoietic stem cells. Recently, we … cells/mL and MLV vector
titers up to 8.6E + 06 transducing … for cell retention and continuous viral vector harvesting. Here, …

[PDF][PDF] A New Method for In Vivo Targeted Gene Transfer into Oligodendrocytes using Adenoviral and HIV Vectors

K Miyake, N Miyake, T Shimada - Biomedical Journal of Scientific & …, 2021 - academia.edu
… specific expression of an ecotropic retroviral receptor gene using Ad and ecotropic retroviral
… two-step gene transfer system with Ad and HIV vectors we could selectively transduce OLs …

Introducing genes into mammalian cells: viral vectors

M Sena-Esteves, G Gao - Cold Spring Harbor Protocols, 2020 - cshprotocols.cshlp.org
… 2-293 cell line sold by Clontech generates ecotropic … allow for selection of retrovirus
vector-transduced cells in culture. (… stem/progenitor cells with retrovirus or lentivirus vectors is …